Short-term variability of bisphenols in spot, morning void and 24-hour urine samples

Abstract Due to worldwide regulations on the application of high production volume industrial chemical bisphenol A (BPA) in various consumer products, alternative bisphenols such as F (BPF) and S (BPS) are increasingly used. To assess human exposure these chemicals, biomonitoring urinary concentrations is frequently However, short-term variability has not been evaluated thoroughly yet, which essential achieve a correct estimation exposure. In this study, we collected all spot urine samples from ten healthy adults for five consecutive days, an additional 24 h pooled sample. We measured seven (BPAF, BPF, BPA, BPB, BPZ, BPS BPAP) using gas chromatography coupled tandem mass spectrometry. BPF were found (>80%), while AP (BPAP) was detected 43% samples. Calculations intra-class correlation coefficients (ICCs) showed that reproducibility four relatively poor (